| 1 | Academy of Notre Dame de Namur | Villanova, Pennsylvania | 22-1-0 | 1 | Inter-AC Champions, PAISAA Champions |
| 2 | The Episcopal Academy | Newtown Square, Pennsylvania | 19-3-0 | 2 | PAISAA Finalist |
| 3 | Emmaus High School | Emmaus, Pennsylvania | 27-1-0 | 4 | District XI AAA Champions, AAA State Champions |
| 4 | Villa Maria Academy | Malvern, Pennsylvania | 22-3-0 | 3 | District 1 AAA Champions, AAA State Semifinalist |
| 5 | Central Bucks West High School | Doylestown, Pennsylvania | 20-5-1 | 14 | District 1 AAA Finalist, AAA State Finalist |
| 6 | The Hill School | Pottstown, Pennsylvania | 14-6-0 | 7 | MAPL Champions, PAISAA Semifinalist |
| 7 | Penn Manor High School | Millersville, Pennsylvania | 19-4-1 | 5 | Lancaster Lebanon League Champions, District 3 AAA Champions, AAA State Semifinalist |
| 8 | Palmyra Area High School | Palmyra, Pennsylvania | 23-3-0 | 8 | District 3 AA Champions, AA State Champions |
| 9 | The Agnes Irwin School | Bryn Mawr, Pennsylvania | 15-5-0 | 10 | PAISAA Semifinalist |
| 10 | Mechanicsburg Area High School | Mechanicsburg, Pennsylvania | 19-5-1 | 9 | District 3 AAA Finalist, AAA State Quarterfinalist |
| 11 | Lower Dauphin High School | Hummelstown, Pennsylvania | 17-5-1 | 6 | District 3 AAA 3rd Place, AAA State Quarterfinalist |
| 12 | Warwick High School | Lititz, Pennsylvania | 22-5-0 | 12 | Lancaster Lebanon League Section 1 Champions, District 3 AA Finalist, AA State Finalist |
| 13 | Downingtown West High School | Downingtown, Pennsylvania | 17-6-0 | 11 | District 1 AAA 4th Place, AAA State First Round |
| 14 | Avon Grove High School | West Grove, Pennsylvania | 22-3-0 | 13 | District 1 AAA 5th Place, AAA State Quarterfinalist |
| 15 | Conestoga High School | Berwyn, Pennsylvania | 16-6-0 | 15 | District 1 AAA 7th Place, AAA State First Round |
| 16 | Parkland High School | Allentown, Pennsylvania | 18-7-0 | 18 | District 11 AAA Finalist, AAA State Quarterfinalist |
| 17 | Manheim Township High School | Lancaster, Pennsylvania | 12-9-1 | OC | District 3 AAA 5th Place, AAA State First Round |
| 18 | Cumberland Valley High School | Mechanicsburg, Pennsylvania | 14-6-2 | 19 | District 3 AAA 6th Place |
| 19 | Springside Chestnut Hill Academy | Philadelphia, Pennsylvania | 11-9-1 | OC | PAISAA Quartefinalist |
| 20 | Mount Saint Joseph Academy | Flourtown, Pennsylvania | 17-8-0 | OC | District 1 AA Finalist, AA State Semifinalist |
| | | | | |
| OC | Boiling Springs High School | Boiling Springs, Pennsylvania | 22-4-1 | NR | District 3 A Champions, A State Finalist |
| OC | Crestwood High School | Mountain Top, Pennsylvania | 17-3-2 | 16 | District 2 AA Champions, AA State Quarterfinalist |
| OC | Easton Area High School | Easton, Pennsylvania | 17-4-1 | OC | District 11 AAA Semifinalist |
| OC | Kennett High School | Kennett Square, Pennsylvania | 19-5-0 | OC | District 1 AA Champions, AA State Quarterfinalist |
| OC | Methacton High School | Eagleville, Pennsylvania | 18-4-2 | 20 | PAC Champions, District 1 AAA Semifinalist, AAA State First Round |
| OC | Susquenita High School | Duncannon, Pennsylvania | 21-2-0 | NR | District 3 A Semifinalist, A State Champions |
2025 PENNSYLVANIA REGION HIGH SCHOOL
